Coding

Part:BBa_K4202015:Design

Designed by: ZIHANG YE   Group: iGEM22_ZJU-China   (2022-09-30)


This part is a fusion protein of EutM and SpyCatcher , and it`s used for the bio-scafford


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to ensure the fucntion of two motives of the fusion protein, a GS linker was utilized for the connection of them.


Source

The main sequence of EutM(GenBank: EDB1220699.1) and the synthetic constructed SpyCatcher(GenBank: AFD50637.1) are obtained from the NCBI. We optimized the codon of the sequence for the protein expression in bacillus subtilis

References